Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum.
نویسندگان
چکیده
Of 1,510 sputum samples examined from 1,016 patients with bronchopulmonary disorders, Nocardia asteroides was isolated from 67 samples by paraffin baiting, as compared with only 30 isolations by the conventional technique of culturing on Sabouraud dextrose agar. This higher efficacy of the paraffin bait technique was found to be statistically significant (P less than 0.001).
منابع مشابه
Comparison of Paraffin Bait, Humic Acid Vitamin B Agar and Paraffin Agar Methods to Isolate Nocardia from Soil
Abstract Background and Objective: The Isolation of Nocardia species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for Nocardia species. Material and Met...
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The principal findings of a study for further evaluating paraffin baiting as a routine laboratory diagnostic procedure in the isolation of Nocardia asteroides, the etiological agent of nocardiosis, are reported.
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Background & Aims: Nocardia are gram-positive, aerobic, relative acid-fast, and opportunistic bacteria, and one of the causes of systematic infection around the world. The natural habitats of these bacteria are soil and dust. Hospitalized patients with immune deficiency are at high risk of transmission of opportunistic infection. Due to these facts and the complexities that exist in the isolati...
متن کاملComparison of Three Methods of Isolation of the Genus Nocardia from the Soil of Hospitals in Isfahan Province, Iran, and its Identification Based on Phenotypic and Molecular Methods
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متن کاملCultivation of Nocardia spp. on chemically defined media for selective recovery of isolates from clinical specimens.
Isolation of Nocardia spp. from clinical specimens can be enhanced by the use of paraffin baiting, which relies on the selective ability of the organism to metabolize paraffin. We evaluated 44 Nocardia isolates, 18 group IV mycobacterial isolates, and 4 Streptomyces isolates for growth on blood agar (BA) and on carbon-free agar containing single or double substrates as follows: paraffin agar (P...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 25 1 شماره
صفحات -
تاریخ انتشار 1987